Cephalotaxus hainanensis li is well known for leukaemia treatment and its endophytic fungi were isolated to investigate the diversity and antimicrobial. Polymerase chain reaction pcr and sequencing were used to identify the isolates, and saponins concentrations in the cultures were measured. A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. Diversity and biological activities of endophytic fungi. Furthermore biodiversity of endophytic fungi in various segments of the plant were. Research article open access diversity of endophytic fungi. Diversity and characterization of endophytic fungi isolated. We focused on the taxonomy and diversity of endophytic fungi associated with the threatened medicinal plant carapichea ipecacuanha, present in a native fragment of the atlantic rain forest in brazil. Diversity of endophytic fungi of paeonia lactiflora pallas. Pdf diversity and characterization of endophytic fungi. Diversity of endophytic fungal community of cacao received.
This chemical diversity also protects host plants from pathogens by inhibiting plant pathogen growth and boosting host immune system to amplify plant defense mechanisms. The species diversity of endophytic fungi isolated from the leaf is higher than that of those isolated from the stem, although the frequency of isolation is lower 42. Diversity of endophytic fungi and screening of fungal. Diversity of endophytic fungal community associated with. Aug 17, 2015 these endophytic fungi showed potentials in medicine development. Elizabeth arnold division of plant pathology and microbiology, department of plant sciences, 1140 e. Review understanding the diversity of foliar endophytic fungi. Fungal endophytes, a diverse group of primarily ascomycetous fungi defined functionally by their occurrence within asymptomatic photosynthetic tissues of plants, occur in all major lineages of land plants and in natural and anthropogenic communities ranging from the arctic to the tropics. Host associations and beta diversity of fungal endophyte. Pdf diversity and antagonistic activity of endophytic fungi. Ravishankar shukla university, raipur, 492010, chhattisgarh, india.
Diversity of endophytic fungi in the roots of mangrove. Diversity studies on the endophytic fungi of vitex negundo l. Copyright mushroom research foundation 2016 diversity of. Tropical and subtropical plants are rich in endophytic community diversity. In a previous study, we assessed the diversity of endophytic fungi from dendrobium orchidaceae and.
A novel proof of concept for capturing the diversity of. Our results indicated that the composition and diversity of endophytic fungi obtained differed using two isolation methods. Research article diversity of endophytic fungi from first. The endophytic fungi and their secondary metabolites have a beneficial role to play in the field of medicine and agriculture. Oct 10, 2002 pdf 146 k pdf plus 235 k citing articles. In recent years, however, endophytic fungi have been gaining increasing interest.
Diversity of endophytic fungi in the roots of mangrove species on the west coast of india. Diverse fungal species live inside plant tissues, some of which presumably occur in a mutualistic association. Diversity and community of culturable endophytic fungi. Diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media zhiqiang xiong1, yingying yang1,2, na zhao1 and yong wang1 abstract background. A total of 128 endophytic fungi were isolated from 29 tissue segments of 14. The aim of this work was the isolation and taxonomic characterization of endophytic fungi from taxus globosa at the sierra alta hidalguense, mexico. Biodiversity and tissuerecurrence of endophytic fungi in tripterygium wilfordii. Fungal endophytes were isolated from healthy plant parts. There is accumulating evidence that plant roots usually harbor mycorrhizal as well as endophytic fungi 29, 30, 34, 39, 52, 63. Introduction endophytic fungi have been studied since 1898 1, but only a limited number of endophytic fungi were reported in the years 18981980s 2. Diversity of endophytic fungi in the roots of mangrove species on the. The majority of studies have focused on angiosperm and conifer hosts and few have examined the endophytes of lycophytes.
Pdf diversity of endophytic fungi of taxus globosa mexican. Endophytic fungi associated with three bryophyte species in the fildes region, king george island, maritime antarctica, that is, the liverwort barbilophozia hatcheri, the mosses chorisodontium aciphyllum and sanionia uncinata, were studied by culturedependent method. Diversity of endophytic fungi associated with the medicinally. Global diversity and geography of soil fungi science. Diversity and ecological distribution of endophytic fungi associated. Endophytes constitute an important component of microbial diversity, and in the present investigation, seven plant species with rich ethnobotanical uses representing six families were analyzed for the presence of endophytic fungi from their natural habitats during monsoon mayjune and winter novemberdecember seasons of 2007. Biodiversity and tissuerecurrence of endophytic fungi in. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Molecular phylogenetic identification of endophytic fungi. In general agreement with biogeographic patterns of plants, animals, and foliar endophytic fungi 5, 32, the overall richness of soil fungi increased toward the equator. Pdf the diversity of endophytic fungi in the aboveground. By continuing to use our website, you are agreeing to our use of cookies.
Variation in endophyte community composition emerging from the interplay of host speci. Some plants of the genus piper are hosts of endophytic. Diversity of fungal endophytes in various tissues of panax. Diversity of endophytic fungi from the ornamental plant adenium obesum. Pdf diversity of endophytic fungi of taxus globosa. Pdf diversity of endophytic fungi isolated from thai medicinal. Pdf endophytic fungi were isolated from casearia grewiifolia and evaluated for antimicrobial activity. The isolates comprised 28 taxa of colletotrichum, ceratobasidium, fusarium.
Diversity and ecological distribution of endophytic fungi. The lowest diversity of endophytic fungi was found in 4yearold american ginseng roots, which may be due to autotoxicity or host defense compounds in the rhizosphere. Plantassociated microorganisms, especially endophytic fungi, are largely underexplored in the discovery of natural products. The enormous diversity of endophytic fungi of the mangrove species was believed to be the major factor. Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, rhizophora mucronata, and identification of potential antagonists against the soilborne fungus. Endophytic fungi have been recognised as sources for new secondary metabolites with useful biological activity. Endophytic fungi harbored in the root of sophora tonkinensis. Although 20 endophytic species were common to more than one age and tissue of the american. High species diversity is another characteristic of endophytic. Copyright mushroom research foundation 2016 diversity. Diversity and taxonomy of endophytic xylariaceous fungi. Cymbidium aloifolium, orchid, endophytic fungi, diversity, extracellular enzymes. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. Endophytic fungi from medicinal plants are an important source of novel and viable drugs.
Highest number of species were isolated from the roots 19 species followed by the stem 15 species and leaf 14 species. Secondly, many endophytic fungi are undescribed and do not fit well into previously described taxa. Interest in fungal endophytes is largely due to their chemical diversity. Dipsacus asperoides contains multiple pharmacologically active compounds. Diversity and characterization of endophytic fungi. Twenty eight strains of endophytic fungi including sterile mycelia were isolated from the. The prolific endophytes also have a capacity to produce diverse class of plant associated secondary metabolites with a wide variety of biological activities such as antimicrobial agent hypericin, acetylcholinesterase inhibitor huperzine a, and. Endophytic fungi from medicinal plant bauhinia forficata. Endophytic fungal diversity in medicinal plants of western. Endophytes, mainly fungi and bacteria, inhabit the healthy plant tissues without causing any damage to the hosts.
Endophytes of medicinal plants have the capacity to synthesis same or similar active substances with their hosts. Srinivasan department of microbiology, dhanalakshmi srinivasan college of arts and science for women, perambalur 621 212, india centre of advanced study in. Culturing of diversity of endophytic fungi from various aegean and mediterranean orchids saleps 442 table 2. Pdf endophytic fungi are microorganisms that live within plant tissues without causing disease during part of their life cycle. Diversity and ecological distribution of endophytic fungi associated with salt marsh plants k. This study aims to lay the foundation for the further study of endophytic fungi from c.
Fiftyfour endophytic fungi with different morphologies were isolated from the leaves, stems and roots of tribulus terrestris l. Endophytic xylariaceous fungi were also recently investigated from many herbs and woody plants, such as liverworts 33, lepanthes and dendrobium orchidaceae 34,35, piper piperaceae 36, pinus and picea pinaceae 37 and mangroves 32. Bmc microbiology diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media zhiqiang xiong 0 yingying yang 0 1 na zhao 0 yong wang 0 0 key laboratory of synthetic biology, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china 1 state key laboratory of. The scale of their diversity, their ecological roles, the insights they provide into the evolution of various ecological modes in fungi, their potential applications, and the ability of many fungi to switch between endophytic and freeliving lifestyles vasiliauskas et al. Diversity and antimicrobial activity of endophytic fungi. Endophytic fungi from medicinal plants wiyakrutta et al. However, major functional and taxonomic groups showed dramatic departures from the general latitudinal richness patterns.
Due to their antifungal and anticancer activity, endophytic fungi have attracted increasing attention, and are considered as promising biological control. A total of 343 endophytic fungal isolates representing 60 taxa including 30 morphotypes were isolated from the different parts of the chinese medicinal plant, tripterygium wilfordii. These endophytic fungi showed potentials in medicine development. The endophytic fungi subjected for production of extracellular enzymes. The identified fungi belonged to the phylum ascomycota. Diversity and taxonomy of endophytic xylariaceous fungi from. To confirm the reliability of morphological identification, all 29. Bmc microbiology diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media zhiqiang xiong 0 yingying yang 0 1 na zhao 0 yong wang 0 0 key laboratory of synthetic biology, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china 1 state key laboratory. Diversity and biological activities of endophytic fungi associated with catharanthus roseus geethanjali dhayanithy, kamalraj subban and jayabaskaran chelliah abstract background. The present study involves diversity and bioactivity of the endophytic fungal community from catharanthus roseus inhabiting the coastal region. Identification of endophytic fungi was carried out using standard taxonomic keys which. Isolation of endophytic fungi the diversity of endophytic fungi was assessed in branches of 3 categories resistant, healthy susceptible and symptomatic of cacao plants.
The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. Diversity of endophytic fungi associated with hedychium spicatum ham ex sm. However, major functional and taxonomic groups showed dramatic departures from the general latitudinal richness patterns fig. Understanding the diversity of foliar endophytic fungi.
One hundred and seventysix fungal isolates were recovered from leaf, stem, and root tissues of c. Diversity of endophytic fungi in paeonia lactiflora pallas, a traditional medicinal herb, and screening for paeoniflorinproducing fungi. Diversity of endophytic fungi associated with carapichea. Endophytes are microorganisms that live within plant tissues without causing symptoms of disease. Diversity of endophytic fungi associated with some medicinal.
Diversity of endophytic fungi associated with some medicinal herbs and shrubs sos in life science, pt. Endophytes are a large and diverse group of fungi that colonize healthy plant tissues without causing any symptoms. The endophytic fungi and their secondary metabolites have a beneficial role. Identity, diversity, and molecular phylogeny of the. Isolates of endophytes and mycorrhizal fungi from aegean and mediterranean orchids. Our results show there is greater species richness of culturable endophytic filamentous fungi in the leaves g. This study suggests that endophytic fungi play an important role in recycling of nutrients in natural ecosystems. Diversity of endophytic fungi associated with hedychium. Diversity and extracellular enzymes of endophytic fungi. We use cookies to enhance your experience on our website. The scale of their diversity, their ecological roles, the insights they provide into the evolution of various ecological modes in fungi, their potential applications, and the ability of many fungi to switch between endophytic and freeliving lifestyles vasiliauskas et. Pdf diversity of endophytic fungi in glycine max researchgate.
Diversity of endophytic fungi in glycine max sciencedirect. Diversity and antifungal activity of endophytic fungi. Mar 25, 2019 the species diversity of endophytic fungi isolated from the leaf is higher than that of those isolated from the stem, although the frequency of isolation is lower 42. Endophytic fungi represent underexplored resource of novel lead compounds and have a capacity to produce diverse class of plant secondary metabolites. Diversity and biological activities of endophytic fungi associated. Association between dipsacus saponin vi level and diversity. Effects of endophytic fungi diversity in different coniferous species.
Diversity of endophytic fungi from various aegean and. A total of 116 fungi were isolated from the bark, branches, leaves and roots of healthy yew trees. Research article open access diversity of endophytic. The colonization frequency cf, species diversity, and species composition of endophytic fungal communities are affected by season, tissue type. Diversity and biological activities of endophytic fungi associated with micropropagated medicinal plant. Diversity of endophytic fungi from root of maize var. Studies in fungi 51, 8493, alternaria solani doi 10. The diversity, antimicrobial and anticancer activity of endophytic fungi associated with the medicinal plant stryphnodendron adstringens mart. Diversity and cold adaptation of culturable endophytic fungi. Endophytic fungi were organisms inhabiting plant organs that at some time in their life. Furthermore biodiversity of endophytic fungi in various segments of the plant were determined by statistical analysis simpson index 1d. Polymerase chain reaction pcr and sequencing were used to identify the isolates, and saponins concentrations in the cultures were. To avoid contamination and to isolate endophytic fungi only from inner plant tissues, the branches were peeled after surface disinfection.
To investigate the diversity and capacity to produce saponins of endophytic fungi of panax ginseng, thirtyeight strains of were isolated. The colonization rate and species richness of endophytic fungi varied in the ten plant species, with higher rates in roots than in stems. These fungi can be useful for biological control of pathogens and plant growth promotion. Antimicrobial and antitumor activity and diversity of. The diversity of endophytic fungal communities in the tissues of plants aboveground as well as belowground, including stems, leaves, andor. A significant part of this research has been directed towards characterization and utilization of the functional diversity of endophytic fungi from traditional medicinal plants, aiming to obtain. Although 20 endophytic species were common to more than one age and tissue of the american ginseng, 7 species were only detected in one age or tissue.
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